Have you never quite been sure about buying 96-well plates for your research? Is this the first time you’ve needed these devices, and want to ensure you buy the best ones possible? If so, then you need some tips for buying 96 healthy plates.
Be sure to look for a company with years of expertise and can offer the best quality and efficiency. Of course, there are several other tips you can look into.
Read on to learn more.
1. Choose the Best Plate Skirt
This refers to the raised edge around each well, which helps prevent cross-contamination between wells. When choosing a plate skirt, it’s essential to look for one that is durable and resistant to scratching or warping. Additionally, consider the height of the dress, which can affect the volume of liquid that can be added to each well.
A shorter skirt may be more convenient for automated liquid handling systems. A taller dress may be better for manual pipetting. Be sure also to consider the material and transparency of the plate skirt, as it can affect the accuracy and clarity of your results.
Depending on the needs of your PCR apparatus, choose between low-profile and high-profile plates. Some tools have movable lids that can be used at either height.
Insufficient contact with the heating block may occur if a high-profile plate is used instead of a low-profile plate, leading to well collapse during amplification.
When a low-profile PCR plate is used with a device that demands a high-profile plate, the lid won’t apply the correct pressure, which will cause sample condensation or evaporation.
2. Pick the Right Plate Height
It is crucial to consider the plate height as it can significantly affect the outcome of your experiments. Determine the maximum depth of your liquid samples. This ensures they do not overflow or evaporate during the incubation process.
Consider the working volume needed for your experiment and choose a plate with appropriate depth. Plates with taller well heights allow for larger books, while shorter well heights are better for smaller volumes.
Skirted and semi-skirted plates have an exterior wall that encircles them to their entire or partial height. Depending on the design of your thermal cycler, decide which skirt choice will best suit your needs. If automation is used, the PCR plate must have a semi- or complete skirt so the robot can grasp it.
It is also essential to consider the compatibility of your plate height with your lab equipment. By carefully considering the plate height, you can ensure accurate and efficient results for your research.
3. Select an Appropriate Color
The color of the plate can play a significant role in your experiments and results. For example, white plates are optimal for fluorescence detection experiments, while clear containers are suitable for colored compounds. It is also essential to consider the material of the scale, as some colors may interact differently with certain chemicals.
Additionally, selecting a color that is easy to distinguish and read results from can save time and minimize errors. Carefully considering the color of your 96-Well plates can significantly impact the accuracy and efficiency of your experiments. Before buying PCR plates, always check your thermal cycler’s specifications, especially for real-time PCR and other fluorophore applications.
Clear wells allow the user to see monitor pipetting from the plate’s bottom, sides, or top. Still, they may also allow fluorescence from nearby wells to leak through, distorting fluorescence readings.
Since white wells are opaque, they offer improved signal-to-noise ratios, which are crucial when monitoring fluorescence for low-abundance targets. However, watching pipetting can be difficult. This flaw is lessened by coloring the sample with an inert dye.
4. Assess Your Rigidity Needs
Consider the assay type you will perform and the required accuracy to determine the appropriate rigidity level. It’s also helpful to read reviews and compare prices. This is to ensure you get the best quality for your budget.
It’s essential to assess your rigidity needs. These plates come in various materials, such as:
Polystyrene
Make sure to check the quality of the material. Polystyrene is a commonly used plastic in lab equipment, but not all brands are created equal. Look for plates made of pure, high-quality polystyrene to ensure accurate and reliable results in your experiments.
Additionally, consider the size and depth of the wells. Different experiments require different healthy sizes. Be sure to choose plates that best fit your needs. Lastly, pay attention to pricing and reviews to ensure you get the best value for your money.
Polypropylene
Look for certified plates free from DNase, RNase, and pyrogen contamination. This will guarantee that your samples will not be affected by any impurities.
Additionally, consider the plate’s chemical resistance, as polypropylene is known to resist many chemicals. It would be best to consider the plate’s optical clarity, as this will affect its performance in assays. Lastly, check for a manufacturer’s warranty and customer reviews to ensure the dish is quality and reliable.
Glass
Consider the type of glass used – borosilicate is generally more durable and chemical-resistant than regular soda-lime glass. Additionally, pay attention to the plate’s compatibility with your specific experiments and lab equipment, and select a container with a clear bottom for optimal visibility.
Remember the wells’ depth and choose a plate that suits your sample size. Lastly, don’t forget to compare prices and quality from different suppliers to find the best deal.
5. Purchase From a Manufacturer You Can Trust
This ensures the product’s quality, reliability, and peace of mind for laboratory experiments. Research and check for customer reviews and feedback to ensure that you buy from a trustworthy manufacturer. Additionally, compare prices and features from different manufacturers to make an informed decision.
Buying directly from the manufacturer is also advisable rather than through a third-party seller. This eliminates any potential risks of counterfeit products.
By purchasing from a trusted manufacturer, you can be confident in the quality of your 96-Well Plates. It focuses on obtaining accurate and reliable results in your research. If you are looking for 96-well plates or a chromatography supplier, visit Chrom Tech.
Choose the Best 96-Well Plates
In conclusion, purchasing 96-well plates can be overwhelming, but these tips can help make the process smoother and more cost-effective. Whether you are a seasoned researcher or a beginner, keeping these factors in mind can ensure you choose the proper plates for your specific needs. So, go ahead and put these tips into practice and make your next 96-well plate purchase a successful one!
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